112 Jenkins-Waggoner Laboratory
The Connecticut Agricultural Experiment Station
New Haven, CT 06511
lindsay.triplett@ct.gov
(203) 974-8611
LABORATORY of LINDSAY TRIPLETT, Ph.D.
BACTERIAL PLANT PATHOLOGY
THE CONNECTICUT AGRICULTURAL EXPERIMENT STATION
EST. 2015
PUBLICATIONS
PEER-REVIEWED
Taerum, S.J., B. Steven, D.J. Gage, and L.R. Triplett. 2020. Validation of a PNA clamping method for reducing host DNA amplification and increasing eukaryotic diversity in rhizosphere microbiome studies. Phytobiomes 4:291-302.
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Read, A.C,, M.J. Moscou, A.V. Zimin, G. Pertea, R.S. Meyer, M.D. Purugganan, J.E. Leach, L.R. Triplett, S.L. Salzberg, and A.J. Bogdanove. 2020. Genome assembly and characterization of a complex zfBED-NLR gene-containing disease resistance locus in Carolina Gold Select rice with Nanopore sequencing. PLOS Genetics 16: e1008571. (Featured cover image).
Karim, S., R.R. McNally, A.S. Nasaruddin, A. Dereeper, R.P. Mauleon, A.O. Charkowski, J.E. Leach, A. Ben-Hur, and L.R. Triplett. 2019. Development of automated primer design workflow Uniqprimer and diagnostic primers for the broad host-range plant pathogen Dickeya dianthicola. Plant Disease 103: 2893-2902.
Shidore, T., Q. Zeng, and L.R. Triplett. 2019. Survey of toxin-antitoxin systems in Erwinia amylovora reveals insights into diversity and functional specificity. Toxins, 11:206.
Peng, J., L.R. Triplett, J. Schachterle, and G. Sundin. 2019. Chromosomally-encoded hok/sok toxin-antitoxin system in the fire blight pathogen Erwinia amylovora: Identification and functional characterization. Applied and Environmental Microbiology 85:e00724-19.
Cui, Z., C.H. Yang, R.R. Kharadi, X. Yuan, G.W. Sundin, L.R. Triplett, J. Wang, and Q. Zeng. 2019. Cell-length heterogeneity: a population-level solution to growth/virulence trade-offs in the plant pathogen Dickeya dadantii. PLoS Pathogens 15: e1007703.
Juanillas, V., A. Dereeper, N. Beaume, G. Droc, J. Dizon, J.R. Mendoza, J.P. Perdon, L. Mansueto, L.R. Triplett, J. Lang, G. Zhou, K. Ratharanjan, B. Plale, J. Haga, J.E. Leach, M. Ruiz, M. Thompson, N. Alexandrov, P. Larmande, T. Kretzschmar, and R.P. Mauleon. 2019. Rice Galaxy: an open resource for plant science. Gigascience 8(5), giz028.
Flores, C., C. Zarate, L.R. Triplett, V. Maillot-Lebon, Y. Moufid, M. Kante, C. Bragard, V. Verdier, L. Gagnevin, B. Szurek, and I. Robene. 2019. Development of a duplex PCR for differential diagnosis of Xanthomonas axonopodis pv. manihotis and Xanthomonas cassavae in cassava (Manihot esculenta). Physiological and Molecular Plant Pathology 105: 34-46.
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Castiblanco, L.F., LR Triplett, and GW Sundin. 2018. Regulation of effector delivery by type III secretion chaperone proteins in Erwinia amylovora. Frontiers in Microbiology 9:146.
Shidore, T., C.D. Broeckling, J.S. Kirkwood, J.J. Long, J. Miao, B. Zhao, J.E. Leach, and L.R. Triplett. 2017. The effector AvrRxo1 phosphorylates NAD in planta. PLOS Pathogens 13: e1006442.
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Leach, J.E., L.R. Triplett, C.T. Argueso, and P. Trivedi. 2017. Communication in the phytobiome. Cell 169: 587-596.
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Read, A.C., F.C. Rinaldi, M. Hutin, Y.-Q. He, L.R. Triplett, and A. J. Bogdanove. 2016. Suppression of Xo1-mediated disease resistance in rice by a truncated, non-DNA-binding TAL effector of Xanthomonas oryzae. Front. Plant Sci., doi: 10.3389/fpls.2016.01516.
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Triplett, L.R., T. Shidore, J. Long, J. Miao, S. Wu, Q. Han, C. Zhou, H. Ishihara, J. Li, B. Zhao, and J.E. Leach. 2016. AvrRxo1 is a bifunctional type III secreted effector and toxin-antitoxin system component with homologs in diverse environmental contexts. PLoS ONE 11: e0158856.
Triplett, L.R, S.P. Cohen, C. Heffelfinger, C.L. Schmidt, C. Tekete, V. Verdier, A.J. Bogdanove, and J.E. Leach. A resistance locus in the American heirloom rice variety Carolina Gold Select is triggered by diverse TAL effectors and is effective against African strains of Xanthomonas oryzae pv. oryzicola. The Plant Journal 87: 472-483.
Publications from graduate and postdoctoral studies:
Langlois, P.A., J. Snelling, J.P. Hamilton, C. Bragard, R. Koebnik, V. Verdier, L.R. Triplett, J. Blom, N.A. Tisserat, and J.E. Leach. 2016. Characterization of the Xanthomonas translucens complex using draft genomes, comparative genomics, phylogenetic analysis, and diagnostic LAMP assays. Phytopathology 107: 519-527.
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Han, Q., C. Zhou, S. Wu, Yi Liu, L.R. Triplett, J. Miao, J. Tokuhisa, L. Deblais, H. Robinson, J.E. Leach, J. Li, and B. Zhao. 2015. Crystal structure of Xanthomonas AvrRxo1-ORF1, a type III effector with a polynucleotide kinase domain, and its interactor AvrRxo1-ORF2. Structure 23:1900-09.
Triplett, L.R, V. Verdier, T. Campillo, C. Van Malderghem, I. Cleenwerk, M. Maes, L. Deblais, R. Corral, O. Koita, B. Cottyn, and J. Leach. 2015. Characterization of a novel clade of Xanthomonas isolated from rice leaves in Mali and proposal of Xanthomonas maliensis sp. nov. Antonie van Leeuwenhoek 107:869-881.
Liu, W., Liu, J., L. Triplett, J.E. Leach, and G.-L. Wang. 2014. Novel insights into rice innate immunity against fungal and bacterial pathogens. Annual Review of Phytopathology 52: 213-241.
Lang, J.M., P. Langlois, M.H.R. Nguyen, L.R. Triplett, L. Purdie, T.A. Holton, A. Djikeng, C.M. Vera Cruz, V. Verdier, and J.E. Leach, 2014. Sensitive detection of Xanthomonas oryzae pv. oryzae and X. oryzae pv. oryzicola by Loop-Mediated Isothermal Amplification. Applied and Environmental Microbiology 80:4519-4530.
Ash, G.J., J.M. Lang, L.R. Triplett, B.J. Stodart, V. Verdier, C. Vera Cruz, P. Rott, and J.E. Leach. 2014. Development of a genomics-based LAMP (Loop-mediated isothermal amplification) assay for detection of Pseudomonas fuscovaginae from rice. Plant Disease 98: 909-919.
Wonni, I., B. Cottyn, L. Detemmerman, S. Dao, L. Ouedraogo, S. Sarra, C. Tekete, S. Poussier, R. Corral, L. Triplett, O. Koita, R. Koebnik, J. Leach, B. Szurek, M. Maes, and V. Verdier. 2014. Analysis of Xanthomonas oryzae pv. oryzicola population in Mali and Burkina Faso reveals a high level of genetic and pathogenic diversity. Phytopathology 104: 520-531.
P.A. Fory, L. Triplett, C. Ballen, J. F. Abello, J. Duitama, M. G. Aricapa, G. A. Prado, F. Correa, J. Hamilton, J. E. Leach, J. Tohme, and G. M. Mosquera. 2014. Comparative analysis of two emerging rice seed bacterial pathogens. Phytopathology 104: 436-444.
Verdier, V.*, L.R. Triplett*, A.W. Hummel, R. Corral, R. Andres Cernadas, C.L. Schmidt, A.J. Bogdanove, and J.E. Leach. 2012. TAL effectors targeting OsSWEET genes enhance virulence on diverse rice varieties when expressed individually in a TAL effector-deficient strain of Xanthomonas oryzae. New Phytologist 196: 1197-1207. (*shared lead author)
Triplett, L., J. Hamilton, C.R. Buell, N.A. Tisserat, V. Verdier, F. Zink, and J.E. Leach. 2011. Genomic analysis of Xanthomonas oryzae from rice grown in the US reveals substantial divergence from known X. oryzae pathovars. Applied and Environmental Microbiology 77: 3930-3937.
Triplett, L., W. Wedemeyer, and G.W. Sundin. 2010. Homology-based modeling of the Erwinia amylovora type III secretion chaperone DspF used to identify amino acids required for virulence and interaction with the effector DspE. Research in Microbiology 161: 613-618.
Triplett, L., M. Melotto, and G.W. Sundin. 2009. Functional analysis of the N terminus of the Erwinia amylovora secreted effector DspA/E reveals features required for secretion, translocation, and binding to the chaperone DspB/F. Molecular Plant-Microbe Interactions 22: 1282-1292.
Triplett, L., Y. Zhao, and G.W. Sundin. 2006. Suppressive subtractive hybridization of blight-causing Erwinia species with differing host specificities. Applied and Environmental Microbiology 72: 7359-64.
CHAPTERS and PROCEEDINGS
Shidore, T. and L.R. Triplett. 2017. Toxin-antitoxin systems: Implications for plant disease. Annual Review of Phytopathology 55: 161-179.
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Triplett, L. and J.E. Leach. "Host mechanisms for resistance to TAL effectors: Thinking outside the UPT box." Proceedings of the 11th US-Japan Scientific Seminar. Physiological and Molecular Plant Pathology 95: 66-69.
Triplett, L., V. Verdier, R. Koebnik, and J.E. Leach. “Genomics of Xanthomonas oryzae.” Genomics of Plant-Associated Bacteria. Eds. D. Gross, A. Lichens-Park, and C. Kole. Springer International, 2014. 127-150.
Triplett, L., M. Melotto, S.-Y. He, and G.W. Sundin. 2008. Translocation and chaperone interaction of the Erwinia amylovora secreted effector DspE. Proceedings of the Eleventh International Workshop on Fireblight. Acta Horticulturae 793: 231-236.